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Chromatographic fingerprint on serially coupled columns combined with multiple-component quantitation with a single reference standard for quality evaluation of Shen-Zhi-Ling oral liquid

Abstract

Conventional liquid chromatographic fingerprint method with a single column lacks enough selectivity and separation power for analyzing complex samples with a wide range of polarities. In this work, a HPLC fingerprint method based on the serial coupling of two columns was developed to evaluate the quality of Shen-Zhi-Ling oral liquid. Twelve batches of Shen-Zhi-Ling oral liquid were used to carry out the fingerprint analysis. Compared to 22 common peaks achieved in fingerprint with a single column, 29 common peaks were obtained when using the serial coupling of two columns under the same conditions. Five components including albiflorin, paeoniflorin, liquiritin, cinnamic acid, and glycyrrhizic acid ammonium salt were simultaneously determined with paeoniflorin as the reference standard. The established method was validated in terms of specificity, linearity, precision, accuracy, stability, and robustness. Then the five components in 12 batches of products were successfully determined. Compared with external standard method, the determination results showed no remarkable differences. In addition, similarity analysis and hierarchical clustering analysis combined with heatmap were also employed to perform quality assessment. The fingerprint based on connecting directly two columns in series combined with multi-component quantitation with single reference standard could provide a feasible and attractive approach to evaluate the quality of Shen-Zhi-Ling oral liquid.

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Supplementary files

Publication details

The article was received on 05 Aug 2018, accepted on 03 Dec 2018 and first published on 03 Dec 2018


Article type: Paper
DOI: 10.1039/C8AY01744A
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Chromatographic fingerprint on serially coupled columns combined with multiple-component quantitation with a single reference standard for quality evaluation of Shen-Zhi-Ling oral liquid

    W. Jiang, X. Liu, M. Su, H. Zang, Y. Zeng and L. Nie, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY01744A

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