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Issue 42, 2018
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Fabrication of an integrated polystyrene microdevice for pre-concentration and amplification of Escherichia coli O157:H7 from raw milk

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Abstract

In this study, we fabricated an integrated polystyrene (PS) microdevice for adhering Escherichia coli O157:H7 by exploiting the pristine PS surface as a pre-concentration step and subsequent amplification using a continuous flow polymerase chain reaction (CF-PCR) for rapid on-site detection. The PS surface, which contained styrene groups, could adhere to bacteria via electrostatic and van der Waals interactions. Subsequently, integration of pre-concentration and amplification using CF-PCR on the PS microdevice was demonstrated for rapid bacterial detection. The introduced method can be an easy-to-use platform for the rapid and simple detection of foodborne pathogens in real samples.

Graphical abstract: Fabrication of an integrated polystyrene microdevice for pre-concentration and amplification of Escherichia coli O157:H7 from raw milk

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Article information


Submitted
31 Jul 2018
Accepted
22 Sep 2018
First published
24 Sep 2018

Anal. Methods, 2018,10, 5071-5077
Article type
Communication

Fabrication of an integrated polystyrene microdevice for pre-concentration and amplification of Escherichia coli O157:H7 from raw milk

K. T. L. Trinh, N. Minh Chon and N. Y. Lee, Anal. Methods, 2018, 10, 5071
DOI: 10.1039/C8AY01707G

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