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Issue 45, 2018
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Lysine detection and quantification by laser desorption/ionization mass spectrometry on gold nanoparticle-enhanced target

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Abstract

Laser desorption/ionization mass spectrometry method on gold nanoparticle enhanced target (AuNPET) was used for rapid detection and quantification of lysine. The amino acid was tested directly in the concentration range from 100 μg mL−1 to 1 ng mL−1, which corresponds to lysine mass of 50 ng to 500 fg per analyzed spot. Detection limit value of 0.2 ng mL−1 or 100 fg per spot is one of the best among the techniques used to determine lysine. In addition, mass spectrometry imaging quantification approach shown in this study canceled out sweet spot effect on the measurement result.

Graphical abstract: Lysine detection and quantification by laser desorption/ionization mass spectrometry on gold nanoparticle-enhanced target

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Publication details

The article was received on 27 Jul 2018, accepted on 21 Oct 2018 and first published on 23 Oct 2018


Article type: Paper
DOI: 10.1039/C8AY01677A
Citation: Anal. Methods, 2018,10, 5398-5405

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    Lysine detection and quantification by laser desorption/ionization mass spectrometry on gold nanoparticle-enhanced target

    A. Arendowski and T. Ruman, Anal. Methods, 2018, 10, 5398
    DOI: 10.1039/C8AY01677A

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