Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 44, 2018
Previous Article Next Article

Chromatographic methods for the determination of acyl-CoAs

Author affiliations


Acyl-CoAs have been identified in various metabolic pathways, such as β-oxidation, biosynthesis and/or remodeling of lipids, fatty acid modification, transcriptional regulation, protein post-translational modification, enzyme regulation, and participation in signaling pathways. Therefore, there is a growing interest in studying these metabolites to grasp a better understanding of how these fatty acid intermediates directly impact these metabolic pathways and induce diseases and cancer progression through their dysregulation. Liquid chromatography coupled with mass spectrometry provides the most robust and reproducible method for the analysis of acyl-CoAs. This review highlights chromatographic methods that have been validated for acyl-CoA determination; however, this review prioritizes LC-MS methods because this approach has recently been utilized more frequently for method development of acyl-CoAs analysis due to its robustness and quantitation applicability. Therefore, the review discusses how current LC-MS methods surpass previous analytical methods for acyl-CoA detection and quantitation, and summarizes the types of mass spectrometers used, preparation approaches for different biological samples, LC conditions for the separation of acyl-CoAs, and potential biomarker applications.

Graphical abstract: Chromatographic methods for the determination of acyl-CoAs

Back to tab navigation

Publication details

The article was received on 03 Jul 2018, accepted on 15 Oct 2018 and first published on 16 Oct 2018

Article type: Minireview
DOI: 10.1039/C8AY01472H
Citation: Anal. Methods, 2018,10, 5252-5264

  •   Request permissions

    Chromatographic methods for the determination of acyl-CoAs

    L. G. Rivera and M. G. Bartlett, Anal. Methods, 2018, 10, 5252
    DOI: 10.1039/C8AY01472H

Search articles by author