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Dual function hollow structured mesoporous Prussian Blue for glucose biosensor


Hollow structured mesoporous materials have attracted great interest in recent years. We present a new glucose biosensor based on dual function hollow structured mesoporous Prussian Blue (PB) mesocrystal (HMPB). HMPB serve as both a scaffold carrier matrix to immobilize the enzyme glucose oxidase (GOx-HMPB) on the electrode and as a redox mediator to H2O2, the by-product of GOx catalyzed glucose reaction that used for glucose detection. The Barrett–Joyner–Halenda (BJH) calculation based on nitrogen adsorption isotherm measurement indicates ~20 nm mesoporous outer shell and a ~60 nm hollow cavity of HMPB that provide a large area (258 m2 g-1) for GOx loading. The larger surface area of HMPB compared to solid PB makes the HMPB much easier to be reduced at lower applied potential (0.130V). The HMPB displays enhanced sensitivity towards glucose detection due to increased GOx loading and increased catalytic sites of HMPB with linear detection range from 0.05 – 7.3 mM, a limit of detection of 0.04 mM (S/N=3) and a fast response time (within 6 s). The detection of glucose level in human serum samples is in good agreement to the data from hospital clinical measurements. The data demonstrate that the HMPB mesocrystal acted effectively as both a redox mediator to H2O2 and a good enzyme carrier, suggesting that the strategy can be applied to other electroactive hollow mesoporous material to prepare simplified biosensors for wide clinical applications.

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Publication details

The article was received on 01 Jul 2018, accepted on 11 Jul 2018 and first published on 12 Jul 2018

Article type: Paper
DOI: 10.1039/C8AY01456F
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Dual function hollow structured mesoporous Prussian Blue for glucose biosensor

    K. Zeng, M. Yang, Y. Liu and A. Rasooly, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY01456F

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