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Simultaneous Detection of Hydrogen and Methane in Breath for the Diagnosis of Small Intestinal Bacterial Overgrowth by Fast Gas Chromatography

Abstract

Hydrogen and methane breath test has been widely used in the diagnosis of SIBO (small intestinal bacterial overgrowth), which is noninvasive and convenient with good accuracy. However, most studies use commercial GC-MS to analyze the breath components with complex operations and high cost, while the most common simplified devices only have single function with some drawbacks. This study proposed a convenient, small-sized and multi-functional fast gas chromatograph (Fast GC) with low detection limit and sufficient resolution to simultaneously determine the hydrogen and methane concentrations and corrected by the carbon dioxide in breath. The system can eliminate the interference of the dilution of outside gas, the patient breathing mode and other factors in the alveolar gas. The detection range of the Fast GC system was 2-200 ppm, and the resolution was 1 ppm, and the precision was less than 10%, and the preheated time was significantly shortened to 2 hours, as well as the size reduced to (40 cm×30 cm×15 cm). Two SIBO diagnostic models were established and forty-seven cases of healthy people and patients were collected and analyzed by our system. This method has the advantages of high specificity, non-invasive and simplicity of operation, so that can be used for the detection of increasing volume of clinical and domestic patients.

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Publication details

The article was received on 30 Jun 2018, accepted on 10 Aug 2018 and first published on 10 Aug 2018


Article type: Paper
DOI: 10.1039/C8AY01451E
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Simultaneous Detection of Hydrogen and Methane in Breath for the Diagnosis of Small Intestinal Bacterial Overgrowth by Fast Gas Chromatography

    F. Gao, M. Wang, X. Zhang, J. Zhang, Y. Xue, H. Wan and P. Wang, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY01451E

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