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Development of a newly and friendly method to evaluate of phenolic compounds from Flos Lonicerae Japonicae by ultra-high performance supercritical fluid chromatography (UHPSFC) combined with chemometrics

Abstract

In recent years, the ultra-high performance supercritical fluid chromatography (UHPSFC) has become a superior technique and powerful analysis tool for the efficient separation of natural products. Chemical variation of 10 batches of Flos Lonicerae Japonicae (FLJ) from different geographical origins was subsequently investigated by quantitation of seven phenolic acids. By studying the effect of different column, temperature, outlet pressure, and flow rates for the separation of seven phenolic acids. Four chromatographic parameters were optimized using central composite design (CCD) test, and a fusion index established by Derringer function was used to evaluate. An ACQUITY UPC2 BEH (100×3.0 mm, 1.7 μm) was selected as the optimal column of the untargeted method. From the CCD test, the optimal values of temperature and outlet pressure were defined as 45°C and 2900 psi, respectively. Our findings confirm that UHPSFC-PDA combined with chemometrics could be used as an efficient method for quality of herbal medicine.

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Supplementary files

Publication details

The article was received on 26 Jun 2018, accepted on 03 Aug 2018 and first published on 09 Aug 2018


Article type: Paper
DOI: 10.1039/C8AY01408F
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Development of a newly and friendly method to evaluate of phenolic compounds from Flos Lonicerae Japonicae by ultra-high performance supercritical fluid chromatography (UHPSFC) combined with chemometrics

    H. Jiang, L. Yang, X. xing, M. Yan, B. yang, L. Yang, M. Cui, Q. Wang and H. Kuang, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY01408F

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