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Fast spectrofluorimetric method for determination of carbinoxamine maleate in Nano-molar range. Application to pharmaceutical preparations, biological fluids and stability studies.

Abstract

Carbinoxamine maleate (CBX), which is a common ingredient of cold and cough treatment preparations, is determined by a sensitive, simple and convenient spectrofluorimetric method whether in its pure form, pharmaceutical preparations and spiked human plasma. This method relies on measuring native fluorescence intensity of CBX in methanol at wavelength 273/308 nm. Furthermore, it was utilized for stability investigation of CBX under stress conditions guided by International Conference of Harmonization (ICH). Until now, no fluorescence-based method is reported for CBX, despite the advantages of this technique. Therefore, we investigated in this study, for the first time, CBX fluorescence behavior to suggest a new spectrofluorimetric method which offers the advantages of simplicity and sensitivity over the previously published reports. The proposed method has a limit of detection of 0.66 ng/mL and a limit of quantitation of 1.99 ng/mL which permits its use in determination of CBX in spiked human plasma samples as a fast and cost-effective tool used for in-vitro investigation with satisfactory results. The method was validated in terms of linearity, accuracy, precision, and robustness and results showed non-significant difference in accuracy and precision, relative to the comparison spectrophotometric method.

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Publication details

The article was received on 27 May 2018, accepted on 11 Jul 2018 and first published on 12 Jul 2018


Article type: Paper
DOI: 10.1039/C8AY01202D
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Fast spectrofluorimetric method for determination of carbinoxamine maleate in Nano-molar range. Application to pharmaceutical preparations, biological fluids and stability studies.

    F. Ali, N. El-Enany, H. Elmansi and A. Nabil, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY01202D

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