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Issue 31, 2018
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A fast spectrofluorimetric method for determination of carbinoxamine maleate in the nano-molar range. Application to pharmaceutical preparations, biological fluids and stability studies

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Abstract

Carbinoxamine maleate (CBX), which is a common ingredient of cold and cough treatment preparations, is determined by a sensitive, simple and convenient spectrofluorimetric method in its pure form, pharmaceutical preparations and spiked human plasma. This method relies on measuring the native fluorescence intensity of CBX in methanol at a wavelength of 273/308 nm. Furthermore, it was utilized for stability investigation of CBX under stress conditions guided by the International Conference of Harmonization (ICH). Until now, no fluorescence-based method has been reported for CBX, despite the advantages of this technique. Therefore, we investigated in this study, for the first time, the CBX fluorescence behavior to suggest a new spectrofluorimetric method which offers the advantages of simplicity and sensitivity over previously published reports. The proposed method has a limit of detection of 0.66 ng mL−1 and a limit of quantitation of 1.99 ng mL−1, which permits its use in the determination of CBX in spiked human plasma samples as a fast and cost-effective tool for in vitro investigation with satisfactory results. The method was validated in terms of linearity, accuracy, precision, and robustness and the results showed a non-significant difference in accuracy and precision, relative to the spectrophotometric comparison method.

Graphical abstract: A fast spectrofluorimetric method for determination of carbinoxamine maleate in the nano-molar range. Application to pharmaceutical preparations, biological fluids and stability studies

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Publication details

The article was received on 27 May 2018, accepted on 11 Jul 2018 and first published on 12 Jul 2018


Article type: Paper
DOI: 10.1039/C8AY01202D
Citation: Anal. Methods, 2018,10, 3851-3858
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    A fast spectrofluorimetric method for determination of carbinoxamine maleate in the nano-molar range. Application to pharmaceutical preparations, biological fluids and stability studies

    F. A. Aly, N. EL-Enany, H. Elmansi and A. Nabil, Anal. Methods, 2018, 10, 3851
    DOI: 10.1039/C8AY01202D

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