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Boronate affinity MIP-based resonance light scattering sensor for sensitive detection of glycoprotein

Abstract

Glycoproteins have extraordinary significance for the diagnosis of tumors or cancers. However, in untreated complex biological samples, highly selective and sensitive determination of glycoproteins is still a huge challenge. In this study, a boronate affinity surface molecular imprinted resonance light scattering (RLS) sensor was constructed to detect glycoproteins. In the RLS sensor, 4-vinylbenzeneboronic acid, which was used as functional monomer for molecular imprinted polymers (MIPs), are pH sensitive and can reversibly bind to glycoproteins. In addition, 2-(Dimethylamino) ethyl methacrylate was also applied as a pH-responsive component to prepare MIPs. The swelling/shrinking property of resultant MIPs is beneficial to rapidly remove template glycoproteins from MIPs. Combining the boronate affinity and MIPs, the RLS sensor can specifically recognize target glycoproteins from sample solutions and sensitively detect them. Under optimum conditions, the RLS sensor has a linear response to ovalbumin (OVA) in the range of 0.5–15 nM with a low detection limit of 0.13 nM. The RLS sensor has been successfully utilized for the detection of OVA in real human blood serum, with good recovery of 94.0%–104%. The approach will provide a promising way to detect glycoproteins in clinical diagnostics.

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Publication details

The article was received on 10 May 2018, accepted on 10 Sep 2018 and first published on 11 Sep 2018


Article type: Paper
DOI: 10.1039/C8AY01053F
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Boronate affinity MIP-based resonance light scattering sensor for sensitive detection of glycoprotein

    Y. Qin, J. Xie, S. Li, C. Cai, G. Zhong, C. Chen and X. Chen, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY01053F

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