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Enzyme-linked immunosorbent assay for aflatoxin B1 using portable pH meter as readout

Abstract

pH meter, one of the most common sensors in the laboratory measurements, has the advantages of excellent sensitivity, low price, high reliability, and ease of use. In this study, the high sensitivity of the pH meter was coupled with the high selectivity of the immunosorbent assay to develop a simple and portable biosensor for aflatoxin B1 (AFB1) determination. Urease was labelled on the AFB1 antigen firstly, in the presence of AFB1, the target competed with urease-labeled AFB1 antigen to be captured by the antibody. Urease could catalyse the hydrolysis of urea into ammonia and induced the pH increasing of the solution, the change could be detected by a handheld pH meter or pH-sensitive dye easily. Under the optimized conditions, the pH value enhancement had a linear relationship with the logarithm of the concentration of AFB1 in the range of 0.62 to 23.42 ng/mL. The proposed biosensor has been applied to detect AFB1 in real samples with satisfied results.

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Publication details

The article was received on 08 May 2018, accepted on 08 Jul 2018 and first published on 09 Jul 2018


Article type: Paper
DOI: 10.1039/C8AY01030G
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Enzyme-linked immunosorbent assay for aflatoxin B1 using portable pH meter as readout

    J. Chen, L. Wang, M. Zhao, D. Huang, F. Luo, L. Huang, B. Qiu, L. Guo, Z. Lin and G. Chen, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY01030G

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