Jump to main content
Jump to site search

Issue 30, 2018
Previous Article Next Article

Enzyme-linked immunosorbent assay for aflatoxin B1 using a portable pH meter as the readout

Author affiliations

Abstract

The pH meter, one of the most common sensors for laboratory measurements, has the advantages of excellent sensitivity, low price, high reliability, and ease of use. In this study, the high sensitivity of the pH meter was coupled with the high selectivity of immunosorbent assay to develop a simple and portable biosensor for aflatoxin B1 (AFB1) determination. Urease was first labelled on the AFB1 antigen, in the presence of AFB1; the target competed with the urease-labeled AFB1 antigen to be captured by the antibody. Urease could catalyse the hydrolysis of urea into ammonia and induce the pH increase of the solution; the change could easily be detected using a handheld pH meter or pH-sensitive dye. Under optimized conditions, the pH value enhancement had a linear relationship with the logarithm of the concentration of AFB1 in the range of 0.62 to 23.42 ng mL−1. The proposed biosensor has been applied to detect AFB1 in real samples with satisfactory results.

Graphical abstract: Enzyme-linked immunosorbent assay for aflatoxin B1 using a portable pH meter as the readout

Back to tab navigation

Publication details

The article was received on 08 May 2018, accepted on 08 Jul 2018 and first published on 09 Jul 2018


Article type: Paper
DOI: 10.1039/C8AY01030G
Citation: Anal. Methods, 2018,10, 3804-3809
  •   Request permissions

    Enzyme-linked immunosorbent assay for aflatoxin B1 using a portable pH meter as the readout

    J. Chen, L. Wang, M. Zhao, D. Huang, F. Luo, L. Huang, B. Qiu, L. Guo, Z. Lin and G. Chen, Anal. Methods, 2018, 10, 3804
    DOI: 10.1039/C8AY01030G

Search articles by author

Spotlight

Advertisements