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Issue 29, 2018
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Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1

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Abstract

In this study, highly fluorescent amino-functionalized CdSe/ZnS core/shell quantum dots (CdSe/ZnS QDs) coated with amphiphilic N-alkylated poly(ethyleneimine) have been synthesized and applied as fluorescent probes in lateral flow immunoassay (LFIA) strips for on-site determination of aflatoxin B1 (AFB1). The amino-functionalized QDs were used to combine with anti-AFB1 via thiol-maleimide conjugation and they effectively enhanced the detection sensitivity. The test strip had high sensitivity for AFB1 from 0.001 ng mL−1 to 10 ng mL−1 with a low limit of detection (LOD) of 0.005 ng mL−1, which was much lower than the allowable concentration by the European Commission (EC) (2.0 μg kg−1) and FDA (20 μg kg−1). The IC50 value was calculated to be 0.025 ng mL−1. The reproducibility and recovery tests showed that the quantitative results were accurate, and it could be used for rapid on-site detection of AFB1 in cereal products.

Graphical abstract: Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1

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Publication details

The article was received on 20 Mar 2018, accepted on 12 Jun 2018 and first published on 19 Jun 2018


Article type: Paper
DOI: 10.1039/C8AY00608C
Anal. Methods, 2018,10, 3582-3588

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    Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1

    J. Li, M. Mao, F. Wu, Q. Li, L. Wei and L. Ma, Anal. Methods, 2018, 10, 3582
    DOI: 10.1039/C8AY00608C

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