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Issue 18, 2018
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One-step detection of hexokinase activity using a personal glucose meter

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Abstract

Hexokinase (HK) is the first key enzyme in metabolic and glycolytic pathways. Aberrations in HK activity may result in various human diseases. In this study, we present a novel, one-step, simple, and rapid method for monitoring HK activity using a commercial personal glucose meter (PGM). In this design, HK catalyzes glucose phosphorylation, forming phosphorylated glucose, which is not detected by a PGM. Accordingly, the amount of unphosphorylated glucose remaining in solution following the HK-catalyzed reaction, which is inversely related to HK activity, can be determined using a PGM. Owing to the favorable features of the PGM, such as its simple operation, small size, low cost, wide accessibility, and portability, this proposed assay has great potential for the point-of-care testing of HK activity.

Graphical abstract: One-step detection of hexokinase activity using a personal glucose meter

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Article information


Submitted
08 Mar 2018
Accepted
09 Apr 2018
First published
10 Apr 2018

Anal. Methods, 2018,10, 2075-2080
Article type
Communication

One-step detection of hexokinase activity using a personal glucose meter

J. Bai, L. Liu, Y. Han, C. Jia and C. Liang, Anal. Methods, 2018, 10, 2075
DOI: 10.1039/C8AY00498F

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