Jump to main content
Jump to site search

Issue 13, 2018
Previous Article Next Article

A simple device for rapid quantification of cell number from equine buccal swab samples

Author affiliations

Abstract

Biological samples are notoriously difficult to use within portable miniaturised devices, often due to complex sample matrices or variation between cellular samples. Herein we describe a device that utilises a correction based upon the total soluble cellular protein and absorbance measurements for the accurate quantification of cell number from equine buccal swabs. Hemocytometer cell counts were initially conducted to determine average mammalian and bacterial contributions (97.66% and 2.34% respectively). Secondly, cells were lysed and centrifuged before the supernatant was utilised within a Bradford's ratio metric absorbance assay using a microplate reader. A correlation coefficient of r = 0.992 was determined, indicating this method was successful in utilising total soluble cellular protein for the quantification of cell number in unknown biological samples of this type. Upon the identification of a strong linear correlation coefficient, a portable microfluidic device was developed (r = 0.983) as a highly specific platform for real time buccal cell number quantification with the ability to be used for sample correction for buccal swab samples as low as 7.28 μg mL−1 in economic downstream biological diagnostic devices.

Graphical abstract: A simple device for rapid quantification of cell number from equine buccal swab samples

Back to tab navigation

Supplementary files

Article information


Submitted
13 Feb 2018
Accepted
23 Feb 2018
First published
26 Feb 2018

Anal. Methods, 2018,10, 1523-1528
Article type
Paper

A simple device for rapid quantification of cell number from equine buccal swab samples

K. Quayle, E. H. Doeven, R. Alexander, Giorgio M. De Guzman, A. F. Clarke, S. J. Haswell, P. S. Francis and X. A. Conlan, Anal. Methods, 2018, 10, 1523 DOI: 10.1039/C8AY00338F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements