Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 15, 2018
Previous Article Next Article

Aptamer-based rolling circle amplification coupled with graphene oxide-based fluorescence resonance energy transfer for sensitive detection of cardiac troponin I

Author affiliations

Abstract

Combining the high affinity and specificity of an aptamer for a protein, a highly sensitive strategy for protein detection was developed based on rolling circle amplification (RCA) and graphene oxide based fluorescence resonance energy transfer (FRET). In this strategy, a protein was captured using an antibody-coated microplate, and then a circularized product containing an aptamer–primer and padlock probe was added to form a sandwich structure. Under the influence of DNA polymerase, the RCA reaction extended the primer sequence, and generated hundreds of copies of the circular DNA template. Detection of the RCA products via FRET using graphene oxide allowed the sensitivity enhancement in target protein detection. This method was used to detect the target protein of cardiac troponin I (cTnI) with a detection limit as low as 14.40 pg mL−1. The highly sensitive and specific assay can be used as a promising tool in clinical sample analysis.

Graphical abstract: Aptamer-based rolling circle amplification coupled with graphene oxide-based fluorescence resonance energy transfer for sensitive detection of cardiac troponin I

Back to tab navigation

Article information


Submitted
08 Feb 2018
Accepted
02 Mar 2018
First published
06 Mar 2018

Anal. Methods, 2018,10, 1767-1773
Article type
Paper

Aptamer-based rolling circle amplification coupled with graphene oxide-based fluorescence resonance energy transfer for sensitive detection of cardiac troponin I

Y. Li, W. Dai, X. Lv and Y. Deng, Anal. Methods, 2018, 10, 1767
DOI: 10.1039/C8AY00309B

Social activity

Search articles by author

Spotlight

Advertisements