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AuNP-peptide probe for Caspase-3 detection in living cells by SERS


Colloidal nanoparticles can be used as a surface enhanced Raman scattering (SERS) substrates because the very close spacing between particles existing in these colloidal system is beneficial for the generation of extremely strong and highly spatially localized electric field enhancements. Herein, a caspase-3 specified peptide was used as molecular cross-linkers to engineer gold nanoparticles (AuNPs)’ junctions in a controllable manner. The peptide was designed with a sequence of CCALNNPFFDVED (Cys-Cys-Ala-Leu-Asn-Asn-Pro-Phe-Phe-Asp-Val-Glu-Asp) or CCALNNKYDDVED (Cys-Cys-Ala-Leu-Asn-Asn-Lys-Tyr-Asp-Asp-Val-Glu-Asp), where the CALNN (Cys-Ala-Leu-Asn-Asn) fragment help to stabilize AuNPs suspension in aqueous media and the sequence of DVED (Asp-Glu-Val-Asp) can be cleaved by caspase-3. And the PFF (Pro-Phe-Phe) or KYD (Lys-Tyr-Asp) was exposed and interacted via the hydrophobic or alternate negative and positive electrointeraction in presence of caspase-3, inducing the aggreation of colloidal Au-peptides accompany with the enhancement of SERS. It can be observed that the SERS enhanced signals were correlated with the caspase-3 concentrations and the limit of detection can be reached to 1.5ng/mL. Finally, this caspase-3 specified AuNPs-peptide probe showed application in analysis of caspase-3 in living cells.

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Publication details

The article was received on 06 Nov 2018, accepted on 28 Nov 2018 and first published on 29 Nov 2018

Article type: Paper
DOI: 10.1039/C8AN02145G
Citation: Analyst, 2018, Accepted Manuscript
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    AuNP-peptide probe for Caspase-3 detection in living cells by SERS

    C. Zhao, P. Lv, L. Qiu, A. Han, G. Fang, J. Liu and S. Wang, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN02145G

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