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A Two-Photon Fluorescent Probe for Basal Formaldehyde Imaging in Zebrafish and Visualization of Mitochondrial Damage Induced by FA Stress

Abstract

Detection of mitochondrial formaldehyde (FA) is of great significance because FA is generated through one-carbon formaldehyde cycle in mitochondria, and aberrant elevations in FA levels can damage mitochondria by decreasing mitochondrial membrane potential and inhibiting mitochondrial respiration. Herein, a mitochondria-targetable two-photon probe (Mito-FA-FP) has been well demonstrated. Mito-FA-FP is conjugated with hydrazine as FA-reactive site and a pyridine derivate as mitochondria-targetable moiety. After reacting with FA, Mito-FA-FP exhibits dramatic fluorescence enhancement (12-fold) due to the block of PET process in probe, and presents good selectivity as well as high sensitivity (LOD: 12.4 uM). Moreover, Mito-FA-FP can be utilized to monitor endogenous FA in mitochondria, and evaluate mitochondrial damage caused by FA stress through observing mitochondrial morphology changes. With good two-photon absorption cross-section and high two-photon fluorescence contrast, Mito-FA-FP has been successfully employed for the two-photon fluorescence imaging of basal FA in zebrafish.

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Publication details

The article was received on 01 Nov 2018, accepted on 23 Nov 2018 and first published on 28 Nov 2018


Article type: Paper
DOI: 10.1039/C8AN02108B
Citation: Analyst, 2018, Accepted Manuscript
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    A Two-Photon Fluorescent Probe for Basal Formaldehyde Imaging in Zebrafish and Visualization of Mitochondrial Damage Induced by FA Stress

    F. Xin, Y. Tian, C. Gao, B. Guo, Y. Wu, J. Zhao, J. Jing and X. Zhang, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN02108B

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