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An electrochemical enzymatic nanoreactor based on dendritic mesoporous silica nanoparticles for living cell H2O2 detection

Abstract

The selective and quantitative detection of cellular H2O2 is essential for understanding its roles in physiology and pathology. A new electrochemical H2O2 biosensor, fabricated by immobilizing horseradish peroxidase onto dendritic mesoporous silica nanoparticles (HRP/DMSNs), is employed for living cell H2O2 detection. Taking advantages of large pore volume and highly accessible internal surface areas of DMSNs, HRP/DMSNs display higher enzymatic loading, better stability and bioactivity in comparison to HRP on nonporous silica nanoparticles (NSNs). Therefore, HRP/DMSNs modified GCE (HRP/DMSNs/GCE) shows attractive electrochemical performance for sensitive and selective detection of H2O2 in 0.1 M pH 7.0 PBS, with low Kmapp value of 11.48 μM and low detection limit of 0.11 μM. In addition, HRP/DMSNs/GCE is successfully applied to detecting H2O2 released from PC12 cell triggered by ascorbic acid (AA). The detected H2O2 amount is close to the reported values. The developed biosensor has the potential in the dynamic detection of the flux of H2O2 from living cells for further evaluation of oxidative stress in cells.

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Supplementary files

Publication details

The article was received on 04 Sep 2018, accepted on 25 Oct 2018 and first published on 31 Oct 2018


Article type: Paper
DOI: 10.1039/C8AN01712C
Citation: Analyst, 2018, Accepted Manuscript
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    An electrochemical enzymatic nanoreactor based on dendritic mesoporous silica nanoparticles for living cell H2O2 detection

    G. Bai, X. xu, Q. Dai, Q. Zheng, Y. Yao, Y. Cheng and S. Liu, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01712C

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