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Analysing Single Live Cells by Scanning Electrochemical Microscopy

Abstract

Single live cell analysis methods provide information on the characteristics of idividual cells, yielding not only bulk population averages but also their heterogeneity. Scanning electrochemical microscopy (SECM) offers single live cell activities along its topograpy with high accuracy probe tip positioning. Both intracellular and extracellular processes can be electrochemically examined through the use of SECM. This non-invasive technique allows for high resolution mapping of electrochemical measurment in or around the cell sample of interest. Reactive oxygen species and reactive nitrogen species can be determined in a non-invasive label-free method and utilized as a probe for cellular pathology and physiology. Membrane permeability and rate of membrane species transport can be quantified in SECM. Cell response to external stressors can be monitored and modelled. SECM is able to offer nanoscale mapping and low concentration detection, providing a powerful bioanalytical tool for live cell studies. Herin we present an overview of recent reasearch progress in the imaging and characterization of single live cells using SECM.

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Publication details

The article was received on 03 Aug 2018, accepted on 16 Sep 2018 and first published on 17 Sep 2018


Article type: Minireview
DOI: 10.1039/C8AN01490F
Citation: Analyst, 2018, Accepted Manuscript
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    Analysing Single Live Cells by Scanning Electrochemical Microscopy

    F. P. Filice and Z. Ding, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01490F

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