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Highly sensitive and multiplexed miRNAs analysis based on digitally encoded silica microparticles coupled with RCA-based cascade amplification

Abstract

Nowadays, miRNAs analysis is significant for not only understanding their regulation networks, but clinical diagnostics and therapy. Analytical strategies feasible for multiplex miRNA sensitive analysis are still highly in demand. Herein, we propose an novel strategy for miRNAs analysis by coupling cascade amplification with digitally encoded silica microparticles. The microparticles precisely fabricated in in a digital form through one-step deposition strategy, is highly efficient for multiplex analysis. The cascade amplification composed of RCA and nicking-assisted strand-displacement amplification (SDA), exhibits high amplification efficiency, requiring no complicated sequence design, thus easy for compatibility with base-stacking hybridization on our microparticles. Parallel and sensitive analysis for let-7a and miR-21 in one pot without mutual interference, has been achieved, with both of high sensitivity (LOD ~ 0.5 fM) and wide dynamic range (10 pM ~ 1 fM). Moreover, our strategy exhibits high specificity for miRNAs of homologous sequence and good anti-interference ability in complex sample matrix. Considering up to 128 (27) kinds of microparticles available, our strategy can be applied for dozens of miRNAs sensitive analysis in one pot, great potential for miRNA signatures analysis as well as widespread clinical applications.

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Supplementary files

Publication details

The article was received on 24 Jul 2018, accepted on 06 Sep 2018 and first published on 11 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN01393D
Citation: Analyst, 2018, Accepted Manuscript
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    Highly sensitive and multiplexed miRNAs analysis based on digitally encoded silica microparticles coupled with RCA-based cascade amplification

    S. Liu, H. Fang, C. Sun, N. Wang and J. Li, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01393D

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