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Naphthalene-based fluorescent probe with large Stokes shift for mitochondrial pH imaging

Abstract

A mitochondrial-targeted pH fluorescent probe 4-(2-(6-hydroxynaphthalen-2-yl)vinyl)-1-methylpyridin-1-ium was facilely synthesized via the carbon-carbon double bond bridging of 6-hydroxy-2-naphthaldehyde and 1,4- dimethylpyridinium iodide salt. The probe exhibited remarkable pH-dependent behavior in the linear range of 7.60-10.00, with a pKa value of 8.85 ± 0.04 near mitochondrial pH. A significantly large Stokes shift of 196 nm was obtained, which reduces the interference of exciting light. Application of probe in live HepG2 cells indicated that the probe had excellently mitochondrial targeting ability and was successfully used to visualize mitochondrial pH fluctuations in live cells.

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Publication details

The article was received on 22 Jul 2018, accepted on 29 Aug 2018 and first published on 31 Aug 2018


Article type: Paper
DOI: 10.1039/C8AN01371C
Citation: Analyst, 2018, Accepted Manuscript
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    Naphthalene-based fluorescent probe with large Stokes shift for mitochondrial pH imaging

    B. Lin, L. Fan, J. Ge, W. Zhang, C. Zhang, C. Dong and S. Shuang, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01371C

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