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Electrochemical aptasensor for zeatin detection based on MoS2 nanosheets and enzymatic signal amplification

Abstract

A simple and sensitive electrochemical aptasensor was constructed for zeatin detection, where MoS2 nanosheets were used as immobilization matrix for gold nanoparticles (AuNPs), and AuNPs were employed as the immobilization matrix to probe DNA. After the aptamer DNA and assist DNA hybridized with probe DNA, the Y-type DNA can be formed with two biotin at the terminals of aptamer DNA. Then, avidin modified alkaline phosphatase (Avidin-ALP) can be further modified on electrode surface through biotin and avidin interaction. Under the catalytic effect of ALP, p-nitrophenylphosphate disodium (PNPP) can be hydrolyzed to produce p-nitrophenol (PNP). However, in the presence of zeatin, the formed Y-type DNA can be destroyed due to the formation of zeatin-aptamer conjugate, which further reduces the amount of PNP and leads to the decrease of the oxidation signal of PNP. Under the optimum conditions, the change of the oxidation peak current of PNP was inversely proportional to the logarithm value of zeatin concentration in the range of 50 pM – 50 nM. The detection limit was calculated to be 16.6 pM. This electrochemical method also showed good detection selectivity and stability. The potential applicability of this method was proved by detecting zeatin in real samples.

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Publication details

The article was received on 20 Jul 2018, accepted on 06 Sep 2018 and first published on 07 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN01356J
Citation: Analyst, 2018, Accepted Manuscript
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    Electrochemical aptasensor for zeatin detection based on MoS2 nanosheets and enzymatic signal amplification

    Y. Zhou, H. Yin, Y. Wang, C. Sui, M. Wang and S. Ai, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01356J

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