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In vivo measurement of dynamics of norepinephrine in olfactory bulb following ischemia-induced olfactory dysfunction and its responses to dexamethasone treatment

Abstract

Information on the dynamics of molecules following olfactory dysfunction remains very essential to understanding molecular events involved in the pathological process of olfactory dysfunction. This study for the first time demonstrates that the combination of in vivo microdialysis with high performance liquid chromatography (HPLC) and electrochemical detection (ECD) enables the measurement of the dynamics of norepinephrine (NE) in olfactory bulb of Sprague-Dawley rats following olfactory dysfunction induced by brain ischemia and its responses toward dexamethasone treatment. The method possesses a high spatial resolution benefited from in vivo microdialysis and high selectivity resulted from HPLC and is thus capable of measuring NE in olfactory bulb of rats. With the method, the basal level of NE in olfactory bulb was evaluated to be ca. 235 ± 25 nM (n = 6). Brain ischemia with bilateral ligation of both common carotid arteries was found to increase the level of NE to 360 ± 90% of the basal level (n = 6) at a time point of 240 min after brain ischemia. This increase was found to be suppressed upon the treatment with 0.2% dexamethasone in olfactory bulb. These results suggest that NE is involved in the pathological process of ischemia-induced olfactory dysfunction and this information would be useful to further understand the molecular events involved in olfactory dysfunction.

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Publication details

The article was received on 14 Jul 2018, accepted on 03 Sep 2018 and first published on 06 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN01300D
Citation: Analyst, 2018, Accepted Manuscript
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    In vivo measurement of dynamics of norepinephrine in olfactory bulb following ischemia-induced olfactory dysfunction and its responses to dexamethasone treatment

    Y. Zhang, L. Li, T. Li, Y. Xin, J. Liu, F. Ma and L. Mao, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01300D

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