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Ratiometric red-emission fluorescent detection of Al3+ in pure aqueous solution and live cells by fluorescent peptidyl probe using aggregation-induced emission

Abstract

The development of a fluorescent method for the selective ratiometric detection of Al3+ ions in pure aqueous solutions and live cells is still a significant challenge. In the present study, we synthesized a new type of a fluorescent probe using an Al3+-triggered self-assembly based on the dipeptide receptor and an aggregation-induced emission fluorophore. The fluorescent probe (1) bearing cyanostilbene with excitation by visible light detected Al3+ ions sensitively in pure aqueous buffered solution by ratiometric red-emission at 600 nm. 1 provided a highly selective ratiometric detection for Al3+ among 16 metal ions in aqueous solution. 1 exhibited sensitive ratiometric response to Al3+ in aqeuous buffered solutions at pH ranging from 5 to 7.4. The detection limit (145 nM, R2 = 0.999) for Al3+ ions in pure aqeuous solution was much lower than the maximum allowable level of Al3+ in drinking water demanded by Environmental Protection Agency (EPA). The probe provided an efficient way to detect low concentrations of Al3+ in ground water, tap water, and live cells by ratiometric red-emissions at 600 nm. The binding study using dynamic light scattering, NMR, IR, and TEM revealed that the complex between 1 and Al3+ self-assembled to form nanoparticles, resulting in the enhancement of the emission at 600 nm and a concomitant decrease in the emission at 535 nm.

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Publication details

The article was received on 04 Jul 2018, accepted on 04 Sep 2018 and first published on 04 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN01221K
Citation: Analyst, 2018, Accepted Manuscript
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    Ratiometric red-emission fluorescent detection of Al3+ in pure aqueous solution and live cells by fluorescent peptidyl probe using aggregation-induced emission

    L. N. Neupane, P. K. Mehta , S. Oh, S. Park and K. Lee, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01221K

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