Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell
This work reports the first images obtained by combining an infrared aperture scanning near-field optical microscope (SNOM) with a quantum cascade laser (QCL). The future potential of this set-up is demonstrated by a preliminary study on an OE33 human oesophageal adenocarcinoma cell in which the cell is imaged at 1751 cm−1, 1651 cm−1, 1539 cm−1 and 1242 cm−1. In addition to the 1651 cm−1 image, three other images were acquired within the Amide I band (1689 cm−1, 1675 cm−1 and 1626 cm−1) chosen to correspond to secondary structures of proteins. The four images obtained within the Amide I band show distinct differences demonstrating the potential of this approach to reveal subtle changes in the chemical composition of a cell.
- This article is part of the themed collections: Clinical spectroscopy and SPEC 2018: International Society of Clinical Spectroscopy