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A novel fluorescent aptasensor for ultrasensitive and selective detection of acetamiprid pesticide based on inner filter effect between gold nanoparticles and carbon dots

Abstract

This paper reports a novel fluorescent aptasensor for the detection of acetamiprid pesticides with high sensitivity and selectivity based on the inner filter effect (IFE) of gold nanoparticles (AuNPs) toward fluorescent carbon dots (CDs). The aptasensor employs S-18 aptamer as the target specific recognition molecule and CDs as the signal transmission element. Free S-18 aptamer sequences can wrap on the surface of AuNPs and make them in a dispersion state even at high amount of Tris-HCl salt, which can effectively quench the fluorescence of subsequent CDs through IFE. Upon adding acetamiprid pesticide, the free S-18 aptamer sequences are firstly exhausted to form some complexes, so that AuNPs tend to be aggregated and its absorption spectrum no longer overlaps with the fluorescence emission spectrum of CDs, which leads to the obvious fluorescence recovery of aptasensor. The properties of CDs were extensively characterized and the fluorescence quenching effect of AuNPs toward CDs was fully investigated. Additionally, the effects of some vital parameters like the type and amount of AuNPs on the fluorescent aptasensor were also investigated. The proposed aptasensor has a detection limit as low as 1.08 µg·L-1 with a line range of 5-100 µg·L-1, and high selectivity against other control pesticides. Moreover, the aptasensor also displays excellent accuracy and recovery in the detection of spiked real samples, suggesting that such fluorescent aptasensor will play an important role in the field of food analysis and environmental detection.

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Publication details

The article was received on 25 Jun 2018, accepted on 08 Sep 2018 and first published on 11 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN01166D
Citation: Analyst, 2018, Accepted Manuscript
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    A novel fluorescent aptasensor for ultrasensitive and selective detection of acetamiprid pesticide based on inner filter effect between gold nanoparticles and carbon dots

    J. Wang, Y. Wu, P. Zhou, W. Yang, H. Tao, S. Qiu and C. Feng, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01166D

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