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Paper-based Transwell assays: an inexpensive alternative to study cellular invasion

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Abstract

Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In this work, we describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix. The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, we quantify invasion: upon addition of an exogenous factor or in the presence of medium obtained from other cell types, in an indirect or direct co-culture format whose medium composition is dynamically changing, and in a single-zone or parallel (96-zone) format.

Graphical abstract: Paper-based Transwell assays: an inexpensive alternative to study cellular invasion

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Publication details

The article was received on 23 Jun 2018, accepted on 09 Oct 2018 and first published on 10 Oct 2018


Article type: Paper
DOI: 10.1039/C8AN01157E
Citation: Analyst, 2018, Advance Article
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    Paper-based Transwell assays: an inexpensive alternative to study cellular invasion

    R. M. Kenney, A. Loeser, N. A. Whitman and M. R. Lockett, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN01157E

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