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Ion mobility mass spectrometry provides novel insights into the expression and structure of gangliosides in normal adult human hippocampus

Abstract

Clustered into the so called „glycosynapses” micodomains in the central nervous system (CNS), gangliosides (GGs) are involved in the formation of functional synapses and neural circuits. Therefore, GGs are important biomarkers in early diagnosis of CNS pathologies, being in the focus of our research as potential therapeutic targets. A series of neuropsychiatric disorders, among which Alzheimer disease and schizophrenia, are characterized by amnesia and disorientation caused by hippocampal atrophy and diminished cholinergic activity. Based on ion mobility mass spectrometry (IM-MS) capability for a reliable determination of glycopatterns, the changes in diversity and number of GGs with age and the occurrence of neurological disorders, we report here on the development of a high performance IM-MS strategy for assessing the GG profile in a complex mixture extracted from a 20 years old hippocampus. IM separation of GGs based on charge state, carbohydrate chain length and degree of sialylation led to the detection and identification of 140 species, the largest number of GGs ever reported before in an adult hippocampus. Moreover, the obtained data support the concept of GG cholinergic activity. IM tandem MS experiments using collision induced dissociation (CID) confirmed the incidence of GD1b(d18:1/24:1) in the investigated hippocampus specimen.

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Publication details

The article was received on 19 Jun 2018, accepted on 06 Sep 2018 and first published on 06 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN01118D
Citation: Analyst, 2018, Accepted Manuscript
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    Ion mobility mass spectrometry provides novel insights into the expression and structure of gangliosides in normal adult human hippocampus

    M. SARBU, Ž. Vukelić, D. E. Clemmer and A. D. Zamfir, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01118D

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