Issue 17, 2018

Analysis of time-course drug response in rat cardiomyocytes cultured on a pattern of islands

Abstract

We previously reported the kinetics analysis of cardiomyocyte beating using scanning electrochemical microscopy (SECM). In this study, a stage-top incubator and a capillary micropipette (MP) for delivering drugs were assembled with an SECM instrument, and the responses of rat cardiomyocytes were analyzed under a culture environment after drug stimulation. When adenosine triphosphate (ATP) was delivered to synchronously beating cardiomyocytes, the beating acceleration effect of ATP was counteracted by the synchronously beating network in the culture dish. In contrast, cardiomyocytes cultured on a pattern of islands in a culture dish showed fluctuations in the duration of beating upon the addition of ATP. We also examined the effect of the cardiotoxic agent astemizole on cardiomyocytes and successfully detected motion fluctuations. Therefore, drug stimulation via MPs and beating measurement by SECM are effective routes for the evaluation of drug candidates through the analysis of time-course beating motion fluctuations of the cardiomyocytes.

Graphical abstract: Analysis of time-course drug response in rat cardiomyocytes cultured on a pattern of islands

Supplementary files

Article information

Article type
Paper
Submitted
05 Jun 2018
Accepted
10 Jul 2018
First published
20 Jul 2018

Analyst, 2018,143, 4083-4089

Analysis of time-course drug response in rat cardiomyocytes cultured on a pattern of islands

S. Okumura, Y. Hirano, Y. Maki and Y. Komatsu, Analyst, 2018, 143, 4083 DOI: 10.1039/C8AN01033A

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