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Real time detection of the nerve agent simulant diethylchlorophosphate by non fluorophoric small molecules generating cyclization induced fluorogenic response

Abstract

Chemical warfare agents (CWA) are some of the most nefarious weapons, and their possible use in terrorist attacks has led to growing interests in the development of reliable and accurate methods to detect these lethal chemicals. In this paper, we have prepared three nonfluorophore containing 2-(2-hydroxybenzylidene)-malononitrile with various 5-substituents as chemosensor probes for a nerve-agent stimulant, diethylchlorophosphate (DCP). The phenolic group of the probes, as the active site, can be rapidly phosphorylated by DCP via nucleophilic substitution and then undergo simultaneous intramolecular cyclization reaction within 45 sec, which can respond to DCP in turn-on fluorescence mode. To the best of our knowledge this is the first report of utilization of non fluorophoric small molecule species to generate fluorogenic response from DCP along with an investigation of substituent effects on sensing response. The detection process can be visualized by the naked eye and in the UV lamp the probe HNBM turns into a strong green fluorescence upon interaction with DCP. Among the three chemosensors, probe HNBM displayed several beneficial attributes such as extremely fast response time along with high selectivity, sensitivity and the lowest limit of detection (0.10 µM) with DCP in solution. TDDFT calculations were performed in order to demonstrate the electronic properties of probe and the cyclized product. Furthermore, the probe was used to develop a low cost portable cellulose paper strip for real-time visual detection of DCP vapor. Also the probe has been extensively used to detect DCP in live-cells.

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Publication details

The article was received on 01 Jun 2018, accepted on 11 Jul 2018 and first published on 12 Jul 2018


Article type: Paper
DOI: 10.1039/C8AN01012A
Citation: Analyst, 2018, Accepted Manuscript
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    Real time detection of the nerve agent simulant diethylchlorophosphate by non fluorophoric small molecules generating cyclization induced fluorogenic response

    S. S. Ali, A. Gangopadhyay, A. K. Pramanik, S. K. Samanta, U. N. Guria, S. Manna and A. K. Mahapatra, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01012A

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