Jump to main content
Jump to site search

Issue 18, 2018
Previous Article Next Article

SEM–Raman image cytometry of cells

Author affiliations

Abstract

Correlative and integrated scanning electron microscopy (SEM) and Raman micro-spectroscopy is presented that enables the characterization and identification of different cancer and non-cancer cells through SEM–Raman image cytometry. The hybrid microscopy system enables the acquisition of high resolution SEM images of uncoated cells and the spatial correlation with chemical information as obtained from Raman micro-spectroscopic imaging. A sample preparation protocol and a workflow are presented that are compatible with the demands of hybrid SEM–Raman microscopy. Stainless steel cell substrates were used that are both conductive and give a low optical response in Raman scattering. Correlative and integrated SEM–Raman micro-spectroscopy is illustrated with cells from blood and cells from a SKBR-3 breast cancer cell line.

Graphical abstract: SEM–Raman image cytometry of cells

Back to tab navigation

Publication details

The article was received on 23 May 2018, accepted on 30 Jul 2018 and first published on 02 Aug 2018


Article type: Paper
DOI: 10.1039/C8AN00955D
Citation: Analyst, 2018,143, 4495-4502
  •   Request permissions

    SEM–Raman image cytometry of cells

    A. Enciso-Martinez, F. J. Timmermans, A. Nanou, L. W. M. M. Terstappen and C. Otto, Analyst, 2018, 143, 4495
    DOI: 10.1039/C8AN00955D

Search articles by author

Spotlight

Advertisements