Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 19, 2018
Previous Article Next Article

A bio-inspired photonic nitrocellulose array for ultrasensitive assays of single nucleic acids

Author affiliations

Abstract

Here we report a bio-inspired photonic nitrocellulose array for ultrasensitive nucleic-acid detection. The patterned photonic nitrocellulose array is inspired by the Stenocara beetle living in the desert, which can collect water on its bumpy back surface from early morning fogs so that spontaneous generation of separated reaction droplets for loop-mediated isothermal amplification (LAMP)-based detection is enabled. Owing to the slow-photon effect of the photonic nitrocellulose, the fluorescence signal of calcein produced during the LAMP reaction can be effectively enhanced (up to 32 fold), which results in dramatically improved sensitivity for the detection of single nucleic acids in 40 min. We demonstrate that Staphylococcus aureus (SA) DNA can be quantitatively detected with a limit-of-detection of 0.60 copy per μL. The consumption of reagents and sample is also remarkably reduced owing to the highly decreased dead volume of the nitrocellulose substrate. Therefore, this bio-inspired photonic nitrocellulose array is promising for carrying out inexpensive, ultrasensitive, and high-throughput nucleic-acid detection under resource-limited settings.

Graphical abstract: A bio-inspired photonic nitrocellulose array for ultrasensitive assays of single nucleic acids

Back to tab navigation

Associated articles

Article information


Submitted
21 May 2018
Accepted
11 Jul 2018
First published
17 Jul 2018

Analyst, 2018,143, 4559-4565
Article type
Paper

A bio-inspired photonic nitrocellulose array for ultrasensitive assays of single nucleic acids

J. Chi, B. Ma, X. Dong, B. Gao, A. Elbaz, H. Liu and Z. Gu, Analyst, 2018, 143, 4559
DOI: 10.1039/C8AN00939B

Social activity

Search articles by author

Spotlight

Advertisements