Jump to main content
Jump to site search


A Multiplex Paper-Based Nanobiocatalytic System for Simultaneous Determination of Glucose and Uric Acid in Whole Blood

Abstract

In this work, a versatile point-of-care assay platform based on a microfluidic paper-based analytic device (μPAD) was developed for simultaneous detection of multiple targets. The μPAD with a central zone and six test zones is fabricated by a simple and inexpensive wax printing method. Flower-like hybrid nanocomplex synthetized with specific dual enzymes and Cu3(PO4)2 inorganic nanocrystals is spotted to the test zones on the μPAD, followed by the introduction of assay targets. Using dual-enzyme inorganic hybrid nanoflowers in the µPADs as nanobiocatalyts, which preserve the activity and enhance the stability for the enzymes, based on the H2O2-mediated catalytic oxidizing chromogenic reaction produced by glucose/uric acid, the developed multiplex paper-based nanobiocatalytic system is demonstrated to enable simultaneous and sensitive detection of glucose and uric acid with the detection limit of 60 and 25 µM, respectively. More importantly, it has been successfully used for detecting glucose and uric acid levels in human whole blood samples. The developed multiplex paper-based nanobiocatalytic system features very easy fabrication and operation, low cost, high sensitivity and have promising prospect for a clinical multianalyte point-of-care test.

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 May 2018, accepted on 28 Jul 2018 and first published on 01 Aug 2018


Article type: Paper
DOI: 10.1039/C8AN00866C
Citation: Analyst, 2018, Accepted Manuscript
  •   Request permissions

    A Multiplex Paper-Based Nanobiocatalytic System for Simultaneous Determination of Glucose and Uric Acid in Whole Blood

    J. huang, X. Zhu, Y. Wang, J. Ge, J. Liu and J. Jiang, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00866C

Search articles by author

Spotlight

Advertisements