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Issue 18, 2018
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A multiplex paper-based nanobiocatalytic system for simultaneous determination of glucose and uric acid in whole blood

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Abstract

In this work, a versatile point-of-care assay platform based on a microfluidic paper-based analytic device (μPAD) was developed for the simultaneous detection of multiple targets. The μPAD with a central zone and six test zones is fabricated by a simple and inexpensive wax printing method. A flower-like hybrid nanocomplex synthesized with specific dual enzymes and Cu3(PO4)2 inorganic nanocrystals is spotted in the test zones on the μPAD, followed by the introduction of assay targets. Using dual-enzyme inorganic hybrid nanoflowers in the μPAD as nanobiocatalysts, which preserve the activity and enhance the stability of the enzymes, based on the H2O2-mediated catalytic oxidizing chromogenic reaction produced by glucose/uric acid, the developed multiplex paper-based nanobiocatalytic system is demonstrated to enable simultaneous and sensitive detection of glucose and uric acid with a detection limit of 60 and 25 μM, respectively. More importantly, it has been successfully used for detecting glucose and uric acid levels in human whole blood samples. The developed multiplex paper-based nanobiocatalytic system features very easy fabrication and operation, low cost, and high sensitivity and has promising prospects for a clinical multianalyte point-of-care test.

Graphical abstract: A multiplex paper-based nanobiocatalytic system for simultaneous determination of glucose and uric acid in whole blood

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Publication details

The article was received on 09 May 2018, accepted on 28 Jul 2018 and first published on 01 Aug 2018


Article type: Paper
DOI: 10.1039/C8AN00866C
Citation: Analyst, 2018,143, 4422-4428
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    A multiplex paper-based nanobiocatalytic system for simultaneous determination of glucose and uric acid in whole blood

    J. Huang, X. Zhu, Y. Wang, J. Ge, J. Liu and J. Jiang, Analyst, 2018, 143, 4422
    DOI: 10.1039/C8AN00866C

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