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Issue 6, 2018
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Current state of bioanalytical chromatography in clinical analysis

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Chromatographic methods have become popular in clinical analysis in both routine and research laboratories. The purpose of this review article is to provide an overview of the current state of chromatographic methods, i.e., high-performance liquid chromatography (HPLC), gas chromatography (GC), and supercritical fluid chromatography (SFC), in clinical analysis. The aspects related to method sensitivity, selectivity, analysis time, and throughput have been discussed in detail. Adequate solutions to improve these features have also been presented. HPLC is the most widely used method among the chromatographic methods, whereas GC is dedicated to several specific applications, and SFC is used only marginally certainly due to its only recent comeback to the analytical scene. Based on the literature search, the application fields in clinical analysis are divided into the following groups: drugs, hormones, drugs of abuse, metabolomics, lipidomics, volatile organic compounds, biomarkers and endogenous compounds, proteomics, multi-analyte approches, and others. The important features of these applications have been emphasized.

Graphical abstract: Current state of bioanalytical chromatography in clinical analysis

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Publication details

The article was received on 05 Nov 2017, accepted on 24 Jan 2018 and first published on 20 Feb 2018

Article type: Critical Review
DOI: 10.1039/C7AN01807J
Citation: Analyst, 2018,143, 1305-1325

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    Current state of bioanalytical chromatography in clinical analysis

    H. Kočová Vlčková, V. Pilařová, P. Svobodová, J. Plíšek, F. Švec and L. Nováková, Analyst, 2018, 143, 1305
    DOI: 10.1039/C7AN01807J

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