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Issue 1, 2018
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Sample preparation for polar metabolites in bioanalysis

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Sample preparation is a primary step of any bioanalytical workflow, especially in metabolomics analysis where maximum information has to be obtained without spoiling the analytical instrument. Because of their biological implication, highly polar metabolites, such as amino acids, nucleobases, and catecholamines seem to attract growing interest in the field of comprehensive metabolomics analysis although their extraction from the matrix remains a real challenge. In this paper, we discuss about the actual practice and issues of hydrophilic metabolites' extraction, including new solutions and perspectives to improve their phase transfer from a complex biological sample to a clean extract prior to analysis.

Graphical abstract: Sample preparation for polar metabolites in bioanalysis

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The article was received on 10 Aug 2017, accepted on 27 Nov 2017 and first published on 27 Nov 2017

Article type: Minireview
DOI: 10.1039/C7AN01333G
Analyst, 2018,143, 16-20

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    Sample preparation for polar metabolites in bioanalysis

    N. Drouin, S. Rudaz and J. Schappler, Analyst, 2018, 143, 16
    DOI: 10.1039/C7AN01333G

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