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Issue 40, 2018
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A DNA nanowire based localized catalytic hairpin assembly reaction for microRNA imaging in live cells

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Abstract

Here, we have developed a localized catalytic hairpin assembly (LCHA) strategy for intracellular miR-21 imaging by using DNA nanowires confining both hairpin probes in a compact space. The LCHA is constructed by interval hybridization of DNA hairpin probe pairs to a DNA nanowire with multiplex footholds generated by alternating chain hybridization. Compared to the conventional catalytic hairpin assembly (CHA) strategy, the LCHA significantly shortens the reaction time and enhances the sensitivity. Moreover, the proposed LCHA can serve as a carrier for delivery of probes into live cells as well as protect the probes from nuclease degradation and enhances the stability. We anticipate that this design can be widely applied in facilitating basic biomedical research and disease diagnosis.

Graphical abstract: A DNA nanowire based localized catalytic hairpin assembly reaction for microRNA imaging in live cells

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Publication details

The article was received on 04 Jul 2018, accepted on 19 Aug 2018 and first published on 20 Aug 2018


Article type: Edge Article
DOI: 10.1039/C8SC02943A
Citation: Chem. Sci., 2018,9, 7802-7808
  • Open access: Creative Commons BY-NC license
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    A DNA nanowire based localized catalytic hairpin assembly reaction for microRNA imaging in live cells

    Q. Wei, J. Huang, J. Li, J. Wang, X. Yang, J. Liu and K. Wang, Chem. Sci., 2018, 9, 7802
    DOI: 10.1039/C8SC02943A

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