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Issue 13, 2018
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Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues

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Abstract

We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly (Drosophila melanogaster) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae.

Graphical abstract: Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues

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Supplementary files

Article information


Submitted
16 Dec 2017
Accepted
26 Feb 2018
First published
26 Feb 2018

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2018,9, 3324-3334
Article type
Edge Article

Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues

G. Lukinavičius, G. Y. Mitronova, S. Schnorrenberg, A. N. Butkevich, H. Barthel, V. N. Belov and S. W. Hell, Chem. Sci., 2018, 9, 3324 DOI: 10.1039/C7SC05334G

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