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Issue 46, 2018, Issue in Progress
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Immunomagnetic separation and size-based detection of Escherichia coli O157 at the meniscus of a membrane strip

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Abstract

We developed a facile method for the detection of pathogenic bacteria using gold-coated magnetic nanoparticle clusters (Au@MNCs) and porous nitrocellulose strips. Au@MNCs were synthesized and functionalized with half-fragments of Escherichia coli O157 antibodies. After the nanoparticles were used to capture E. coli O157 in milk and dispersed in a buffer solution, one end of a test strip was dipped into the solution. Due to the size difference between the E. coli–Au@MNC complexes (approximately 1 μm) and free Au@MNCs (approximately 180 nm), only E. coli–Au@MNC complexes accumulated at the meniscus of the test strip and induced a color change. The color intensity of the meniscus was proportional to the E. coli concentration, and the detection limit for E. coli in milk was 103 CFU mL−1 by the naked eye. The presence of E. coli–Au@MNC complexes at the meniscus was confirmed using a real-time PCR assay. The developed method was highly selective for E. coli when compared with Salmonella typhimurium, Listeria monocytogenes, and Staphylococcus aureus.

Graphical abstract: Immunomagnetic separation and size-based detection of Escherichia coli O157 at the meniscus of a membrane strip

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Supplementary files

Article information


Submitted
03 Jun 2018
Accepted
18 Jul 2018
First published
23 Jul 2018

This article is Open Access

RSC Adv., 2018,8, 26266-26270
Article type
Paper

Immunomagnetic separation and size-based detection of Escherichia coli O157 at the meniscus of a membrane strip

H. Lee, J. Hwang, Y. Park, D. Kwon, S. Lee, I. Kang and S. Jeon, RSC Adv., 2018, 8, 26266
DOI: 10.1039/C8RA04739A

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