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Issue 11, 2018
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Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

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Abstract

We have created modified protein variants by introducing a non-canonical amino acid p-azido-L-phenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b562 proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability.

Graphical abstract: Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

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Publication details

The article was received on 10 Oct 2017, accepted on 11 Jan 2018 and first published on 05 Feb 2018


Article type: Paper
DOI: 10.1039/C7RA11166E
RSC Adv., 2018,8, 5768-5775
  • Open access: Creative Commons BY license
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    Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

    A. J. Zaki, Andrew M. Hartley, S. C. Reddington, S. K. Thomas, P. Watson, A. Hayes, A. V. Moskalenko, M. F. Craciun, J. E. Macdonald, D. D. Jones and M. Elliott, RSC Adv., 2018, 8, 5768
    DOI: 10.1039/C7RA11166E

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