Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 10, 2018
Previous Article Next Article

Biosynthesis and isolation of selenoneine from genetically modified fission yeast

Author affiliations

Abstract

Selenoneine, a naturally occurring form of selenium, is the selenium analogue of ergothioneine, a sulfur species with health relevance not only as a purported antioxidant but likely also beyond. Selenoneine has been speculated to exhibit similar effects. To study selenoneine's health properties as well as its metabolic transformation, the pure compound is required. Chemical synthesis of selenoneine, however, is challenging and biosynthetic approaches have been sought. We herein report the biosynthesis and isolation of selenoneine from genetically modified fission yeast Schizosaccharomyces pombe grown in a medium containing sodium selenate. After cell lysis and extraction with methanol, selenoneine was purified by three consecutive preparative reversed-phase HPLC steps. The product obtained at the mg level was characterised by high resolution mass spectrometry, NMR and HPLC/ICPMS. Biosynthesis was found to be a promising alternative to chemical synthesis, and should be suitable for upscaling to produce higher amounts of this important selenium species in the future.

Graphical abstract: Biosynthesis and isolation of selenoneine from genetically modified fission yeast

Back to tab navigation

Supplementary files

Article information


Submitted
24 Jul 2018
Accepted
17 Sep 2018
First published
17 Sep 2018

Metallomics, 2018,10, 1532-1538
Article type
Paper

Biosynthesis and isolation of selenoneine from genetically modified fission yeast

N. G. Turrini, N. Kroepfl, K. B. Jensen, T. C. Reiter, K. A. Francesconi, T. Schwerdtle, W. Kroutil and D. Kuehnelt, Metallomics, 2018, 10, 1532
DOI: 10.1039/C8MT00200B

Social activity

Search articles by author

Spotlight

Advertisements