Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 8, 2018
Previous Article Next Article

Ultra-rapid rates of water splitting for biohydrogen gas production through in vitro artificial enzymatic pathways

Author affiliations

Abstract

Unlocking the potential of the hydrogen economy requires breakthroughs in production, storage, distribution, and infrastructure. Here we demonstrate an in vitro artificial enzymatic pathway that can produce hydrogen at extremely high rates by splitting water energized by carbohydrates (e.g., starch). This fifteen hyperthermophilic enzymes pathway comprises ATP-free starch phosphorylation, an NAD-based pentose phosphate pathway, and a biomimetic electron transport chain consisting of a diaphorase, an electron mediator benzyl viologen (BV), and a [NiFe]-hydrogenase, whereas fast electron transfer was facilitated by utilizing a BV-conjugated diaphorase. The highest reaction rate of 530 mmol H2 per L per h was accomplished at 80 °C. Two NAD-conjugated dehydrogenases were further applied to enable nine-day hydrogen production with a total turnover number of NAD of over 100 000, along with hyperthermophilic enzymes. This biohydrogen production system characterized by the highest chemical-energy efficiency and an exceptionally-high reaction rate addresses challenges associated with cost-effective, distributed hydrogen production, off-board hydrogen storage, and infrastructure.

Graphical abstract: Ultra-rapid rates of water splitting for biohydrogen gas production through in vitro artificial enzymatic pathways

Back to tab navigation

Supplementary files

Article information


Submitted
15 Mar 2018
Accepted
08 May 2018
First published
08 May 2018

Energy Environ. Sci., 2018,11, 2064-2072
Article type
Paper
Author version available

Ultra-rapid rates of water splitting for biohydrogen gas production through in vitro artificial enzymatic pathways

E. Kim, J. Kim and Y. P. J. Zhang, Energy Environ. Sci., 2018, 11, 2064
DOI: 10.1039/C8EE00774H

Social activity

Search articles by author

Spotlight

Advertisements