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Issue 12, 2018
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Bioinspired construction of multi-enzyme catalytic systems

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Enzyme catalysis, as a green, efficient process, displays exceptional functionality, adaptivity and sustainability. Multi-enzyme catalysis, which can accomplish the tandem synthesis of valuable materials/chemicals from renewable feedstocks, establishes a bridge between single-enzyme catalysis and whole-cell catalysis. Multi-enzyme catalysis occupies a unique and indispensable position in the realm of biological reactions for energy and environmental applications. Two complementary strategies, i.e., compartmentalization and substrate channeling, have been evolved by living organisms for implementing the complex in vivo multi-enzyme reactions (MERs), which have been applied to construct multi-enzyme catalytic systems (MECSs) with superior catalytic activity and stabilities in practical biocatalysis. This tutorial review aims to present the recent advances and future prospects in this burgeoning research area, stressing the features and applications of the two strategies for constructing MECSs and implementing in vitro MERs. The concluding remarks are presented with a perspective on the construction of MECSs through rational combination of compartmentalization and substrate channeling.

Graphical abstract: Bioinspired construction of multi-enzyme catalytic systems

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Supplementary files

Article information

31 Dec 2017
First published
08 May 2018

Chem. Soc. Rev., 2018,47, 4295-4313
Article type
Tutorial Review

Bioinspired construction of multi-enzyme catalytic systems

J. Shi, Y. Wu, S. Zhang, Y. Tian, D. Yang and Z. Jiang, Chem. Soc. Rev., 2018, 47, 4295
DOI: 10.1039/C7CS00914C

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