Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 45, 2018
Previous Article Next Article

The hitchhiker's guide to quantitative diffusion measurements

Author affiliations

Abstract

Quantifying diffusional motion of submicron tracer particles with high spatiotemporal resolution is often key to assess the dynamics of physico-chemical systems, e.g. in complex fluids, colloidal suspensions, or living cells. A variety of methods has been developed over the past decades, but often their quantitative comparability has remained poorly explored on an experimental basis. Yet, knowing their experimental benefits and limitations can be a crucial piece of information when designing experiments on new and unexplored specimen. Therefore, we have implemented three very widespread techniques for quantifying diffusional motion (single-particle tracking, fluorescence correlation spectroscopy, and differential dynamic microscopy) in a light sheet microscope and performed a quantitative comparison on standardized samples of varying concentrations. Light sheet microscopy is particularly suited for imaging-based diffusion measurements because of its high spatiotemporal resolution in combination with an improved contrast and signal-to-noise-ratio at low excitation powers. As a result, all three methods are found to yield good quantitative estimates with respect to the theoretically predicted diffusion constant, yet their accuracy and bias varies markedly, raising specific caveats for the individual methods' range of applicability.

Graphical abstract: The hitchhiker's guide to quantitative diffusion measurements

Back to tab navigation

Article information


Submitted
02 Oct 2018
Accepted
05 Nov 2018
First published
06 Nov 2018

Phys. Chem. Chem. Phys., 2018,20, 28910-28919
Article type
Paper

The hitchhiker's guide to quantitative diffusion measurements

P. Struntz and M. Weiss, Phys. Chem. Chem. Phys., 2018, 20, 28910
DOI: 10.1039/C8CP06158K

Social activity

Search articles by author

Spotlight

Advertisements