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Issue 98, 2018
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Dual enzyme-assisted one-step isothermal real-time amplification assay for ultrasensitive detection of polynucleotide kinase activity

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Abstract

A novel, simple, one-step and one-tube detection method was developed for ultrasensitive detection of polynucleotide kinase (PNK) activity on the basis of dual enzyme-synergistic signal amplification. This method was also demonstrated to work well for PNK inhibitor screening and endogenous PNK detection in cell lysates at a single-cell level.

Graphical abstract: Dual enzyme-assisted one-step isothermal real-time amplification assay for ultrasensitive detection of polynucleotide kinase activity

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Article information


Submitted
28 Oct 2018
Accepted
16 Nov 2018
First published
16 Nov 2018

Chem. Commun., 2018,54, 13841-13844
Article type
Communication

Dual enzyme-assisted one-step isothermal real-time amplification assay for ultrasensitive detection of polynucleotide kinase activity

X. Li, Y. Du, Y. Pan, L. Su, S. Shi, S. Wang, A. Tang, K. Kim and D. Kong, Chem. Commun., 2018, 54, 13841
DOI: 10.1039/C8CC08616H

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