Issue 99, 2018
From the journal:

Chemical Communications

Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes†‡

Yoshihide Tokunou, ORCID logo a   Punthira Chinotaikul,a   Shingo Hattori,b   Thomas A. Clarke,c   Liang Shi,d   Kazuhito Hashimoto,e   Kazuyuki Ishii ORCID logo b  and  Akihiro Okamoto*e  

* Corresponding authors

a Department of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

b Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8605, Japan

c Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, UK

d Department of Biological Sciences, School of Environmental Studies, The China University of Geosciences, Wuhan, Hubei 430074, P. R. China

e International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
E-mail: okamoto.akihiro@nims.go.jp

Abstract

We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics.

Graphical abstract: Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

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Article information

DOI
https://doi.org/10.1039/C8CC06309E
Article type
Communication
Submitted
02 Aug 2018
Accepted
12 Sep 2018
First published
07 Nov 2018
This article is Open Access
Creative Commons BY license

Chem. Commun., 2018,54, 13933-13936

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Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

Y. Tokunou, P. Chinotaikul, S. Hattori, T. A. Clarke, L. Shi, K. Hashimoto, K. Ishii and A. Okamoto, Chem. Commun., 2018, 54, 13933 DOI: 10.1039/C8CC06309E

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