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Issue 49, 2018
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XNA ligation using T4 DNA ligase in crowding conditions

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Abstract

T4 DNA ligase is capable of ligating 2′OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before.

Graphical abstract: XNA ligation using T4 DNA ligase in crowding conditions

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Supplementary files

Article information


Submitted
26 Mar 2018
Accepted
30 May 2018
First published
31 May 2018

Chem. Commun., 2018,54, 6408-6411
Article type
Communication

XNA ligation using T4 DNA ligase in crowding conditions

D. Kestemont, M. Renders, P. Leonczak, M. Abramov, G. Schepers, V. B. Pinheiro, J. Rozenski and P. Herdewijn, Chem. Commun., 2018, 54, 6408
DOI: 10.1039/C8CC02414F

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