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Issue 38, 2018
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Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification†

Yongpeng Yao,‡ad   Shanshan Li,‡b   Jiaqian Cao,ad   Weiwei Liu,ad   Keqiang Fan, ORCID logo a   Wensheng Xiang,b   Keqian Yang,*a   Deming Kong ORCID logo *c  and  Weishan Wang ORCID logo *a  
Author affiliations

* Corresponding authors

a State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. China
E-mail: wangws@im.ac.cn, Yangkq@im.ac.cn

b State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China

c State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin, 300071, P. R. China
E-mail: kongdem@nankai.edu.cn

d University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Abstract

Here, we demonstrate an easy-to-implement and general biosensing strategy by coupling the small-molecule recognition of the bacterial allosteric transcription factor (aTF) with isothermal strand displacement amplification (SDA) in vitro. Based on this strategy, we developed two biosensors for the detection of an antiseptic, p-hydroxybenzoic acid, and a disease marker, uric acid, using bacterial aTF HosA and HucR, respectively, highlighting the great potential of this strategy for the development of small-molecule biosensors.

Graphical abstract: Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification

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Supplementary files

Article information

https://doi.org/10.1039/C8CC01764F
Submitted
04 Mar 2018
Accepted
08 Mar 2018
First published
10 Mar 2018
Chem. Commun., 2018,54, 4774-4777
Article type
Communication
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Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification

Y. Yao, S. Li, J. Cao, W. Liu, K. Fan, W. Xiang, K. Yang, D. Kong and W. Wang, Chem. Commun., 2018, 54, 4774
DOI: 10.1039/C8CC01764F

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