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Issue 17, 2018
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Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

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Abstract

The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplification in minimally buffered conditions producing, as a readout, pH shifts attaining subunits of pH.

Graphical abstract: Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

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Article information


Submitted
19 Dec 2017
Accepted
02 Feb 2018
First published
02 Feb 2018

Chem. Commun., 2018,54, 2158-2161
Article type
Communication

Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

E. Tenaglia, Y. Imaizumi, Y. Miyahara and C. Guiducci, Chem. Commun., 2018, 54, 2158
DOI: 10.1039/C7CC09609G

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