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Issue 11, 2018
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‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes

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Abstract

β(1,4)-Galactosyltransferase (β4Gal-T1) and T. cruzi trans-sialidase (TcTS) have been used in a ‘one-pot’ cascade to provide vesicles (liposomes) with a trisaccharide coating. These soluble enzymes catalysed the transfer of galactose then sialic acid onto a synthetic N-acetylglucolipid embedded in the bilayers. Clustering of this substrate into microdomains increased the rate of sialylated lipid production, showing that an increase in β4Gal-T1 activity is carried through the enzymatic cascade. These coatings modulated cell recognition. Hepatocellular carcinoma cells took up vesicles modified by β4Gal-T1 alone more extensively than sialylated vesicles produced by ‘one-pot’ sequential enzymatic modification.

Graphical abstract: ‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes

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Supplementary files

Article information


Submitted
28 Nov 2017
Accepted
04 Jan 2018
First published
04 Jan 2018

Chem. Commun., 2018,54, 1347-1350
Article type
Communication

‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes

F. L. Craven, J. Silva, M. D. Segarra-Maset, K. Huang, P. Both, J. E. Gough, S. L. Flitsch and S. J. Webb, Chem. Commun., 2018, 54, 1347
DOI: 10.1039/C7CC09148F

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